Detection of AD-BMP-2-IRES-HIF-1α MU on local promoting angiogenic and osteogenic capacity of necrosis area

• Published in: Pakistan journal of pharmaceutical sciences Vol. 30; no. 5(Supplementary); pp. 2013 - 2019
• Main Authors: Cui, Fuai, Wang, Xiuli, Wang, Wei, Xiao, Peng, Ma, Yuan, Jiang, Lindong
• Format: Journal Article
• Language: English
• Published: Pakistan 01.09.2017
• Subjects: Adenoviridae - genetics; Animals; Bone Morphogenetic Protein 2 - genetics; Bone Morphogenetic Protein 2 - metabolism; Cells, Cultured; Disease Models, Animal; Endothelial Progenitor Cells - metabolism; Endothelial Progenitor Cells - transplantation; Female; Femur Head Necrosis - genetics; Femur Head Necrosis - metabolism; Femur Head Necrosis - physiopathology; Femur Head Necrosis - therapy; Genetic Therapy - methods; Hypoxia-Inducible Factor 1, alpha Subunit - genetics; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Internal Ribosome Entry Sites - genetics; Male; Neovascularization, Physiologic; Osteogenesis; Preliminary Data; Rabbits; Signal Transduction; Stem Cell Transplantation - methods; Time Factors; Transfection
• ISSN: 1011-601X

The induced EPCs were transfected by Ad-BMP-2-IRES-HIF-1αmu, and then transplanted into femoral head necrotic zone, the effect on osteogenesis and agiogenesis of necrosis zone was detected. The Ad-BMP-2-IRES-HIF-1α was transfected into induced EPCs and then transplanted into avascular necrotic parts of the femoral head (ANFH).Afterwards, the promotion effect on angiogenic and osteogenic capabilities of the necrosis parts from Ad-BMP-2-IRES-HIF-1α was detected. Rabbit bone marrow MNCs were obtained by density gradient centrifugation method, and were induced into EPCs by M199 medium; EPCs were identified in accordance with the cell morphology, specific surface markers and uptake abilities. The Ad-BMP-2-IRES-HIF-1α was transfected to EPCs and then transplanted into parts of ANFH. The models were euthanized 2 and 4 weeks after operation and then the angiogenic and osteogenic indexes of necrotic parts were detected. The results showed that more blood vessels generated in group A than that in group B and C (P<0.05), and the statistical differences were found between group B and C (P<0.05). The detection of histology and BMP-2 immunohistochemistry showed that there were statistically significant differences between group A and B, group A and C (P<0.05). There was no significant difference between group B and C (P<0.05). To sum up, this experiment shows that the EPCs transfected by Ad-BMP-2-IRES-HIF-1α have stronger angiogenic and osteogenic capabilities.