Qualification and quantification of 10 sulfonamides in animal feedstuff by high performance liquid chromatography-electrospray tandem mass spectrometry

The presence of sulfonamide (SA) residues in foods is largely due to the raising of animals with sulfonamide antibiotics added or polluted feedstuff. Because of interference from the matrices, the commonly used immunoassay or chromatographic method is not suitable for the analysis of multi-SAs in fe...

Full description

Saved in:
Bibliographic Details
Published inSepu Vol. 23; no. 4; p. 397
Main Authors Qin, Yan, Zhang, Meijin, Lin, Haidan
Format Journal Article
LanguageChinese
Published China 01.07.2005
Subjects
Animal Feed - analysis
Animals
Chromatography, High Pressure Liquid - methods
Spectrometry, Mass, Electrospray Ionization
Sulfadimethoxine - analysis
Sulfadimethoxine - chemistry
Sulfameter - analysis
Sulfameter - chemistry
Sulfamethazine - analysis
Sulfamethazine - chemistry
Sulfamethoxazole - analysis
Sulfamethoxazole - chemistry
Sulfamethoxypyridazine - analysis
Sulfamethoxypyridazine - chemistry
Sulfamonomethoxine - analysis
Sulfamonomethoxine - chemistry
Sulfapyridine - analysis
Sulfapyridine - chemistry
Sulfaquinoxaline - analysis
Sulfaquinoxaline - chemistry
Sulfonamides - analysis
Sulfonamides - chemistry
Tandem Mass Spectrometry - methods
Online AccessGet more information

Cover

More Information
Summary:The presence of sulfonamide (SA) residues in foods is largely due to the raising of animals with sulfonamide antibiotics added or polluted feedstuff. Because of interference from the matrices, the commonly used immunoassay or chromatographic method is not suitable for the analysis of multi-SAs in feedstuff. A high performance liquid chromatographic-electrospray tandem mass spectrometric (HPLC/ESI-MS-MS) method has been established for the simultaneous determination of multi-SAs including sulfadiazine (SD), sulfapyridine (SPD), sulfamerazine (SM1), sulfameter (SM), sulfamethazine (SM2), sulfamethoxypyridazine (SMP), sulfamethoxazole (SMZ), sulfamonomethoxine (SMM), sulfadimethoxine (SDM) and sulfaquinoxaline (SQX). After solvent extraction, solid phase extraction, dilution and reversed-phase HPLC separation, SAs were detected by ESI-MS-MS under multi-reaction monitoring mode. The qualification analysis was done by using retention time and distribution of diagnostic ion pairs, and the quantification was based on the peak intensity of common fragment ion m/z 156. The limits of quantification for 10 SAs were 0.5 - 2.0 microg/kg (S/N = 10). The correlation coefficient of linear calibration curve was over 0.9995 within the SAs concentration range 2.0 - 200 microg/L except for SDM and SQX. At the spiked level of 1.0 mg/kg, the average recoveries for the 10 SAs were between 70% and 92%, the relative standard deviations were under 10% for intra-day and under 15% for inter-day. Routine tests showed the method was fast, sensitive, specific, and practical for the SAs determination in feedstuff.
ISSN:1000-8713