Effects of isoflurane on Nfκb p65, Gadd45a and Jnk1 expression in the vital organs of CBA/CA mice

• Published in: In vivo (Athens) Vol. 25; no. 2; pp. 241 - 244
• Main Authors: Kádár, Balázs, Gombos, Katalin, Szele, Eszter, Ember, István, Iványi, János László, Csejtei, Rás, Pajkos, Gábor
• Format: Journal Article
• Language: English
• Published: Greece 01.03.2011
• Subjects: Anesthetics, Inhalation - pharmacology; Animals; Cell Cycle Proteins - genetics; Gene Expression Regulation - drug effects; Humans; Isoflurane - pharmacology; Kidney - drug effects; Kidney - metabolism; Liver - drug effects; Liver - metabolism; Lung - drug effects; Lung - metabolism; Mice; Mice, Inbred CBA; Mitogen-Activated Protein Kinase 8 - genetics; Nuclear Proteins - genetics; Reverse Transcriptase Polymerase Chain Reaction; Time Factors; Transcription Factor RelA - genetics
• ISSN: 1791-7549

Isoflurane is administered to patients during general anaesthesia to diminish the effects of surgical invasion. CBA/CA mice were exposed to 2% isoflurane during general anaesthesia. Gene expressions were measured 6 and 12 hours later by quantitative real-time PCR on total RNA isolated from the lung, liver and kidneys of the animals. Expressions of growth arrest and DNA-damage-inducible 45 alpha (Gadd45 α), Jun N-terminal kinase 1 (Jnk1) and nuclear factor of kappa light polypeptide gene enhancer in B-cells p65 subunit (Nfκb p65) were analyzed. Gadd45α and Jnk1 showed significant inverse expression changes in the different tissues compared to the Nfκb p65. The length of anaesthesia also modified the gene induction. Isoflurane has significant modulatory effect on the transcription of genes regulating inflammation and apoptotic signalling.