Synthesis of sulfur-contained D(+)-glucosamine metal complexes and interaction with DNA and serum albumin

• Published in: Guang pu xue yu guang pu fen xi Vol. 28; no. 2; p. 374
• Main Authors: Wu, Shan-Shan, Zhang, Yin-Feng, Du, Jin-Feng, Zhang, Qi, Yuan, Wen-Bing, Gu, Hai-Bo
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2008
• Subjects: Animals; Cattle; DNA - chemistry; Glucosamine - chemistry; Humans; Hydrogen-Ion Concentration; Metals, Heavy - chemistry; Serum Albumin - chemistry; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Sulfur Compounds - chemical synthesis; Sulfur Compounds - chemistry
• ISSN: 1000-0593

Four sulfur-contained D(+)-glucosamine metal complexes were synthesized (M-GLUS, M = Co, Cu, Ni and Zn)and characterized by elementary analysis, molar conductance, and proton nuclear magnetic resonance. The yield of the complex was about 70%, and it dissolved easily in water. The ligand coordinates with metalions mainly between sulphur and metalions, the coordination molar ratio of the ligand to metalion was 2 : 1, and the molar conductivity indicated that all the complexes are nonelectrolyte. The mechanism of the interaction between metal complexes and calf thymus (ct) DNA in Tris buffer (pH = 7. 08), was studied by ultraviolet absorption and fluorescence spectroscopy. The results from varied experiments showed that the intensity of the maximal absorption peaks increased with gradual addition of metal complexes, but the metalions can decrease the maximal absorption and the ligand has no effect on it. Meanwhile, metal complexes could remarkably quench the emission intensity of the DNA-EB system, and the metal