One year variability of peak heights, heterozygous balance and inter-locus balance for the DNA positive control of AmpFℓSTR© Identifiler© STR kit

• Published in: Forensic science international : genetics Vol. 5; no. 1; pp. 43 - 49
• Main Authors: Debernardi, A, Suzanne, E, Formant, A, Pène, L, Dufour, A B, Lobry, J R
• Format: Journal Article
• Language: English
• Published: Netherlands 01.01.2011
• Subjects: Alleles; Cell Line, Transformed; Cell Transformation, Viral; Control Groups; DNA - genetics; DNA Fingerprinting - methods; Genetic Loci; Genotype; Herpesvirus 4, Human - physiology; Heterozygote; Humans; Microsatellite Repeats - genetics; Multivariate Analysis; Nucleic Acid Amplification Techniques - methods; Polymerase Chain Reaction; Reagent Kits, Diagnostic; Retrospective Studies; Sensitivity and Specificity
• ISSN: 1878-0326
• DOI: 10.1016/j.fsigen.2010.01.020

Multivariate analyses of 205 positive control experiments in an AmpFℓSTR© Identifiler© STR kit were used to analyze the factors affecting peak heights at 16 loci. Peak heights were found to be highly correlated between loci and there was evidence for a difference in sensitivity of the two genetic analyzers in the blue channel. Heterozygous balance response at 10 loci was found to behave as a random variable following a beta-distribution with typical median values of 90%, without locus or genetic analyzer effect. Inter-locus balance at 16 loci was influenced by the blue channel effect and a temporal switch of unexplained origin. The implications of these results for the choice of minimum threshold values in quality control are discussed.