Determination of papaverine in seeds of Papaver somniferum L. and soup of chafing dish by high performance liquid chromatography-fluorescence detection

A method of reversed-phase high performance liquid chromatography coupled with fluorescence detection was developed for the separation and determination of papaverine in seeds of Papaver somniferum L. and soup of chafing dish. The liquid chromatographic experiment was carried out under the following...

Full description

Saved in:
Bibliographic Details
Published inSepu Vol. 23; no. 6; p. 639
Main Authors Pi, Li, Hu, Fengzu, Shi, Zhixian
Format Journal Article
LanguageChinese
Published China 01.11.2005
Subjects
Chromatography, High Pressure Liquid - methods
Drugs, Chinese Herbal - analysis
Drugs, Chinese Herbal - chemistry
Papaver - chemistry
Papaverine - analysis
Papaverine - chemistry
Reproducibility of Results
Seeds - chemistry
Spectrometry, Fluorescence - methods
Online AccessGet more information

Cover

More Information
Summary:A method of reversed-phase high performance liquid chromatography coupled with fluorescence detection was developed for the separation and determination of papaverine in seeds of Papaver somniferum L. and soup of chafing dish. The liquid chromatographic experiment was carried out under the following conditions: the detector was a fluorescence with excitation wavelength at 285 nm and emission wavelength at 355 nm. An RP-C18 column (250 mm x 4.6 mm i.d., 5 microm) was used. The mobile phase was a mixture of methanol and 0.02 mol/L ammonium acetate (70: 30, v/v). The flow rate was 0.8 mL/min. A linear range was obtained from 0.1 ng to 0.1 microg with a good correlation. The lowest detection limit was 0.02 ng. The average recovery of seeds for Papaver somniferum L. was 99.0% - 100.8%. The method is rapid, accurate, sensitive and suitable for the separation and determination of papaverine in foods.
ISSN:1000-8713