Preparation of three capsaicinoid components using preparative high performance liquid chromatography

A method was established for the isolation of three capsaicinoid components from capsaicinoids using preparative high performance liquid chromatography (Pre-HPLC). A preparative PRC-ODS column (250 mm x 21.5 mm, 13 microm) was used in the preparation process. By isocratic elution with methanol-water...

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Bibliographic Details
Published inSepu Vol. 26; no. 3; p. 366
Main Authors Dong, Xinrong, Liu, Zhonghua, Li, Benxiang, Wang, Kunbo
Format Journal Article
LanguageChinese
Published China 01.05.2008
Subjects
Analytic Sample Preparation Methods - methods
Capsaicin - analysis
Capsaicin - chemistry
Capsaicin - isolation & purification
Chromatography, High Pressure Liquid - methods
Reproducibility of Results
Solvents - chemistry
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Summary:A method was established for the isolation of three capsaicinoid components from capsaicinoids using preparative high performance liquid chromatography (Pre-HPLC). A preparative PRC-ODS column (250 mm x 21.5 mm, 13 microm) was used in the preparation process. By isocratic elution with methanol-water (70 : 30, v/v) as the mobile phase at a flow rate of 15 mL/min followed by concentration, three capsaicinoid components were obtained from 80% capsaicinoids, and subsequently identified by 1H NMR (nuclear magnetic resonance) and EI-MS (electron impact-mass spectrometry) as nordihydrocapsaicin, capsaicin and dihydrocapsaicin, and the purities of the three components analyzed by HPLC were 98.12%, 99.93% and 100.7% with yields of 60.1%, 58.9% and 72.3%, respectively.
ISSN:1000-8713