Signaling molecules for early detection of adverse interactions during mechanical ventilation in animal models

• Published in: In vivo (Athens) Vol. 25; no. 2; pp. 209 - 217
• Main Authors: Kobr, J, Pizingerova, K, Fremuth, J, Sasek, L, Kocova, J, Hes, O, Racek, J, Topolcan, O
• Format: Journal Article
• Language: English
• Published: Greece 01.03.2011
• Subjects: Animals; Biomarkers - blood; Disease Models, Animal; Immunoassay - methods; Intercellular Adhesion Molecule-1 - blood; Interleukin-6 - blood; Lung - pathology; Lung - physiopathology; Lung - ultrastructure; Lung Injury - blood; Lung Injury - diagnosis; Lung Injury - etiology; Microscopy, Electron, Transmission; Multiple Organ Failure - blood; Multiple Organ Failure - diagnosis; Multiple Organ Failure - etiology; Natriuretic Peptide, Brain - blood; Respiration, Artificial - adverse effects; Sensitivity and Specificity; Swine; Time Factors; Tumor Necrosis Factor-alpha - blood; Vascular Cell Adhesion Molecule-1 - blood
• ISSN: 1791-7549

The early identification of adverse interactions during mechanical ventilation, investigated by multiplexed immunoanalysis. Twenty piglets (average age 7 weeks, weight 23 kg) were intubated and divided into groups: A, spontaneously breathing; B, protectively ventilated; C, ventilated with injurious strategy; D, ventilated with lung disability. At the 1st hour (time-1) and 12th hour (time-2) of the study, brain natriuretic peptide (BNP), intercellular cell adhesion molecules (ICAM-1), vascular cell adhesion molecules (VCAM-1), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (Il-6) were analyzed in the blood. The injurious ventilated group C exhibited an increase in both cell adhesion molecules (p<0.01), TNF-alpha and BNP (p<0.05) at time-1, and at time-2 further increases (p<0.05). In group D, an increase in ICAM-1 and BNP (p<0.05) at time-1, and increases in Il-6 and ICAM-1 (p<0.05) at time-2, with notable decreases in urine output were observed. Overall, the lung damage correlated with TNF-alpha (r=0.904), Il-6 (r=0.740), and ICAM-1 (r=0.756) levels. All five monitored molecules quickly and reliably signaled adverse interactions.