Raman spectra and structure analysis of 2,6-pyridine dicarboxylic acid in different states and single Bacillus spore

• Published in: Guang pu xue yu guang pu fen xi Vol. 31; no. 3; p. 681
• Main Authors: Huang, Rong-shao, Huang, Xi, Xu, Lan-lan, Li, Yong-qing, Huang, Shu-shi
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2011
• Subjects: Bacillus - chemistry; Optical Tweezers; Picolinic Acids - chemistry; Spectrum Analysis, Raman - methods; Spores, Bacterial - chemistry
• ISSN: 1000-0593
• DOI: 10.3964/j.issn.1000-0593(2011)03-0681-06

The Raman spectra of 2,6-pyridine dicarboxylic acid (DPA) and their calcium salts(Ca-DPA) in different states and the Ca-DPA in a single bacterial spore have been recorded by laser tweezers Raman system (LTRS) and the spectra have been assigned. Raman spectra of different states of DPA and Ca-DPA are different evidently. Analysis leading to differences in the structure of spectrum may be due to that the Raman spectra of DPA crystalline reflected more precise characteristics information compared to DPA powder, in which the laser can penetrate through DPA crystalline and the Raman scatter from the crystalline interior is greater than that from DPA powder. The second reason is that DPA powder and Ca-DPA crystalline contain water molecules, and the intermolecular hydrogen bonding in the crystals of these molecules is extensive. The presence of calcium ions would affect the pyridine ring so that both sides of the carboxyl pyridine ring have a certain geometric deformation and the hydroxy carboxylic was damaged. Th