Effects of purified glycerol from biodiesel on Cyp1a1 and Cyp2e1 expressions in CBA/CA mice

• Published in: In vivo (Athens) Vol. 25; no. 2; pp. 237 - 240
• Main Authors: Szele, Eszter, Gombos, Katalin, Kovács, András, Ember, István
• Format: Journal Article
• Language: English
• Published: Greece 01.03.2011
• Subjects: Administration, Oral; Animals; Biofuels; Cytochrome P-450 CYP1A1 - genetics; Cytochrome P-450 CYP2E1 - genetics; Female; Gene Expression Regulation, Enzymologic - drug effects; Glycerol - administration & dosage; Glycerol - pharmacology; Liver - drug effects; Liver - metabolism; Male; Mice; Mice, Inbred CBA; Reverse Transcriptase Polymerase Chain Reaction; Time Factors; Up-Regulation - drug effects
• ISSN: 1791-7549

The glycerol from biodiesel as a valuable by-product can be used as food supplements in feed for animals. We investigated the effect of this by-product on metabolism in mice. CBA/CA mice were administered purified glycerol products (called SZME3) for 3, 6 and 24 hours. After dietary exposure, gene expressions of cytochrome P450, family 1, subfamily a polypeptide 1 (Cyp1a1) and cytochrome P450, family 2, subfamily e, polypeptide 1 (Cyp2e1) were analysed in liver. SZME3 induced an up-regulation of both genes in both genders after three hours' administration. After longer administration of SZME3, the expression of genes became similar to that of the controls. Based upon our data SZME3 has no long-term effect on oxidative metabolism, it seems not to cause oxidative stress for liver cells.