Investigation on damage of bovine serum albumin (BSA) catalyzed by nano-sized silicon dioxide (SiO2) under ultrasonic irradiation using spectral methods

• Published in: Guang pu xue yu guang pu fen xi Vol. 29; no. 4; p. 1069
• Main Authors: Wang, Jun, Ding, Na, Zhang, Zhao-hong, Guo, Ying, Wang, Shi-xian, Xu, Rui, Zhang, Xiang-dong
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2009
• Subjects: Animals; Catalysis; Cattle; Metal Nanoparticles - chemistry; Oxidation-Reduction; Particle Size; Serum Albumin, Bovine - chemistry; Silicon Dioxide - chemistry; Spectrometry, Fluorescence - methods; Ultrasonics
• ISSN: 1000-0593
• DOI: 10.3964/j.issn.1000-0593(2009)04-1069-05

The damage of bovine serum albumin (BSA) molecules under ultrasonic irradiation in the presence of nano-sized silicon dioxide (SiO2) particles was studied by UV-Vis and fluorescence spectra. In addition, the influences of ultrasonic irradiation time, nano-sized SiO2 addition amount, solution acidity (pH) and ultrasonic irradiation power on the damage of BSA molecules in aqueous solution were also detected. For BSA solution of 1.0 x 10(-5) mol x L(-1) at (37.0+/-0.2) degrees C, the UV-Vis spectra of BSA solutions showed that the absorption peaks of BSA displayed obvious hyperchromic effect with the increase in some influence factors such as ultrasonic irradiation time, nano-sized SiO2 addition amount, pH value and ultrasonic irradiation power. However, the fluorescence spectra of BSA solutions showed the phenomenon of fluorescence quenching with the increase in ultrasonic irradiation time, nano-sized SiO2 addition amount, pH value and ultrasonic irradiation power. Moreover, the possible mechanism behind the da