Isolation of hydrocarbon degrading bacteria from soils contaminated with crude oil spills

A feasibility study was conducted to evaluate the capability of bacterial strains to degrade crude oil under in vitro conditions. Pseudomonas strain PS-I could degrade alkanes (70.69%) and aromatics (45.37%). Alkanes and aromatic fractions separated by column chromatography were analyzed by gas chro...

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Bibliographic Details
Published inIndian journal of experimental biology Vol. 47; no. 9; p. 760
Main Authors Mittal, Anupama, Singh, Padma
Format Journal Article
LanguageEnglish
Published India 01.09.2009
Subjects
Biodegradation, Environmental
Feasibility Studies
Hydrocarbons - chemistry
Hydrocarbons - metabolism
Petroleum - metabolism
Pseudomonas - classification
Pseudomonas - isolation & purification
Pseudomonas - metabolism
Soil Microbiology
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Summary:A feasibility study was conducted to evaluate the capability of bacterial strains to degrade crude oil under in vitro conditions. Pseudomonas strain PS-I could degrade alkanes (70.69%) and aromatics (45.37%). Alkanes and aromatic fractions separated by column chromatography were analyzed by gas chromatography. In case of Pseudomonas strain PS-I, nC17/Pr, nC18/Ph ratios decreased from 2.5100 to 0.1232 and from 7.2886 to 0.3853, respectively. It was concluded that out of the isolated strains, Pseudomonas strain PS-I, PS-II and PS-III were comparatively better and potent hydrocarbon degraders. Pseudomonas strain PS-I was almost comparable with standard strain of Acinetobacter calcoaceticus in crude oil biodegradation potency.
ISSN:0019-5189