Frequency of hepatitis C viral RNA in anti-hepatitis C virus non- reactive blood donors with normal alanine aminotransferase

• Published in: Journal of the College of Physicians and Surgeons--Pakistan Vol. 20; no. 10; pp. 662 - 666
• Main Authors: Ali, Nadir, Moinuddin, Ahmed, Syed Azhar, Chotani, Rashid A, Fisher-Hoch, Susan P
• Format: Journal Article
• Language: English
• Published: Pakistan 01.10.2010
• Subjects: Adult; Alanine Transaminase - blood; Blood Donors; Enzyme-Linked Immunosorbent Assay; Hepacivirus - genetics; Hepacivirus - immunology; Hepacivirus - isolation & purification; Hepatitis B Surface Antigens - blood; Hepatitis C Antibodies - blood; Humans; Male; Middle Aged; RNA, Viral - blood; Young Adult
• ISSN: 1022-386X
• DOI: 10.2010/JCPSP.662666

To determine the frequency of HCV RNA in an anti-HCV non-reactive blood donor population with normal ALT, and its cost effectiveness. An observational study. Baqai Institute of Haematology, Baqai Medical University, Karachi, and Combined Military Hospital, Malir Cantt, Karachi, from May 2006 to April 2008. After initial interview and mini-medical examination, demographic data of blood donors was recorded, and anti-HCV, HBsAg and HIV were screened by third generation ELISA. Those reactive to anti-HCV, HbsAg and/or HIV were excluded. Four hundred consecutive donors with ALT within the reference range of 15-41 units/L were included in study. HCV RNA RT-PCR was performed on 5 sample mini-pools using Bio-Rad Real time PCR equipment. All 400 donors were male, with mean age 27 years SD + 6.2. ALT of blood donors varied between 15-41 U/L with mean of 31.5+6.4 U/L, HCV RNA was detected in 2/400 (0.5%) blood donors. Screening one blood bag for HCV RNA costs Rs 4,000.00 equivalent to 50 US dollars, while screening through 5 sample mini-pools was Rs. 800.00 equivalent to approximately 10 US dollars. HCV RNA frequency was 0.5% (2/400) in the studied anti-HCV non-reactive normal ALT blood donors. Screening through mini-pools is more cost-effective.