Comparison of spectrophotometric, HPLC and chemiluminescence methods for 3-nitrotyrosine and peroxynitrite interaction

We have studied the interaction of 3-nitrotyrosine with peroxynitrite using three different methods; chemiluminescence, spectrophotometry and HPLC. Peroxynitrite-induced luminol or lucigenin chemiluminescence were significantly decreased by 3-nitrotyrosine, in concentration-dependent manners. The in...

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Published inArchives of pharmacal research Vol. 28; no. 3; pp. 358 - 363
Main Authors Turan, Nilufer Nermin, Ark, Mustafa, Demiryurek, Abdullah Tuncay
Format Journal Article
LanguageEnglish
Published Korea (South) 01.03.2005
Subjects
Chromatography, High Pressure Liquid
Hypochlorous Acid - chemistry
Luminescent Measurements
Peroxynitrous Acid - chemistry
Spectrophotometry, Ultraviolet
Tyrosine - analogs & derivatives
Tyrosine - chemistry
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Summary:We have studied the interaction of 3-nitrotyrosine with peroxynitrite using three different methods; chemiluminescence, spectrophotometry and HPLC. Peroxynitrite-induced luminol or lucigenin chemiluminescence were significantly decreased by 3-nitrotyrosine, in concentration-dependent manners. The intensity of the peroxynitrite spectrum was also markedly reduced in the presence of 3-nitrotyrosine in the spectrophometric assay. However, there was no attenuation of the 3-nitrotyrosine signal in the HPLC assay after mixing with peroxynitrite. The interaction of 3-nitrotyrosine and hypochlorous acid (HOCl) was also studied via the chemiluminescence assay, where the HOCl-induced responses were markedly inhibited by 3-nitrotyrosine. These results suggest that caution should be taken when studying the levels or interactions of 3-nitrotyrosine.
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ISSN:0253-6269
DOI:10.1007/BF02977805