Apolipoprotein E polymorphism in Turkish subjects with Type 2 diabetes mellitus: allele frequency and relation to serum lipid concentrations

The role of apolipoprotein E (apoE) genotypes in modulating plasma lipid and apolipoprotein levels was studied in 112 patients with Type 2 diabetes mellitus (T2DM) and 94 healthy individuals. ApoE genotypes were identified by PCR amplification and subsequent restriction endonuclease digestion. The a...

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Bibliographic Details
Published inDiabetes, nutrition & metabolism Vol. 17; no. 5; p. 267
Main Authors Duman, B Süsleyici, Oztürk, M, Yilmazer, S, Hatemi, H
Format Journal Article
LanguageEnglish
Published Italy 01.10.2004
Subjects
Analysis of Variance
Apolipoproteins - blood
Apolipoproteins E - blood
Apolipoproteins E - genetics
Blood Glucose - analysis
Body Mass Index
Case-Control Studies
Diabetes Mellitus, Type 2 - blood
Diabetes Mellitus, Type 2 - genetics
DNA - chemistry
Female
Gene Frequency
Genotype
Humans
Lipids - blood
Male
Middle Aged
Polymerase Chain Reaction
Polymorphism, Genetic
Restriction Mapping
Sex Distribution
Triglycerides - blood
Turkey
Turkey
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Summary:The role of apolipoprotein E (apoE) genotypes in modulating plasma lipid and apolipoprotein levels was studied in 112 patients with Type 2 diabetes mellitus (T2DM) and 94 healthy individuals. ApoE genotypes were identified by PCR amplification and subsequent restriction endonuclease digestion. The apoE allele and genotype frequencies were similar in both the diabetic and control subjects. The apoE allele frequencies were found to be 74.3 for e3, 10.1 for e2, 15.6 for e4 in the diabetic group, and 68.1 for e3, 13.2 for e2 and 18.7 for e4 in the control group. Sex-specific genotypic distribution of apoE polymorphism did not differ between the study groups. To elucidate the association of apoE with lipid abnormalities with respect to gender, serum lipid and apolipoprotein levels were compared among apo e2 (e2/2 and e3/2), e3 (e3/3) and e4 (e4/3 and e4/4) groups of T2DM and control subjects. Apo e2 allele was found to be associated to triglycerides for both sexes, and associated to glucose, and BMI only in females. Subjects with e2 allele had higher levels of BMI, glucose and triglyceride in comparison to e3 and e4. Our data suggest that genetic variation at the apoE locus in Turkish subjects is a genetic factor that influences lipid levels. Further studies attempting to correlate apoE polymorphism with lipid profile in a large number of individuals would be helpful in establishing the true significance of this polymorphism in the Turkish population.
ISSN:0394-3402