Fluorescence characteristics and photophysical parameters of aggregates of light-harvesting chlorophyll a/b complexes

Changes in the fluorescence characteristics and molecular photophysical parameters induced by disaggregation of light-harvesting chlorophyll a/b complexes isolated from pea were studied. Disaggregation was achieved by increasing the concentration of the detergent triton X-100 (concentration range 0-...

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Bibliographic Details
Published inBiofizika Vol. 52; no. 5; p. 855
Main Authors Ostroumov, E E, Fadeev, V V, Khristin, M S, Pashchenko, V Z, Tusov, V B
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.09.2007
Subjects
Chlorophyll - chemistry
Chlorophyll A
Cold Temperature
Light-Harvesting Protein Complexes - chemistry
Octoxynol - chemistry
Pisum sativum - chemistry
Plant Proteins - chemistry
Spectrometry, Fluorescence
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Summary:Changes in the fluorescence characteristics and molecular photophysical parameters induced by disaggregation of light-harvesting chlorophyll a/b complexes isolated from pea were studied. Disaggregation was achieved by increasing the concentration of the detergent triton X-100 (concentration range 0-230 microM, chlorophyll concentration 2.45 microg/ml). Laser fluorimetry methods were used to measure the molecular photophysical parameters. It was shown that the decrease in aggregate size is accompanied by a decrease in fluorescence at 700 nm at 77 K, a fall of the singlet-singlet annihilation rate (by more than two orders), and the growth of fluorescence life time (from 160 ps to 3.2 ns).
ISSN:0006-3029