Molecular reasons for the regulation of the enzymatic activity of bovine pancreatic deoxyribonuclease I as studied by dynamic light scattering and fluorescent spectroscopy

The effect of catalytic bivalent and inhibitory monovalent cations on the interactions of bovine pancreatic deoxyribonuclease I with the circle single-stranded DNA of M13 phase was studied. It was found that monovalent cations affect the site of binding to DNA and the active center of the enzyme; in...

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Bibliographic Details
Published inBiofizika Vol. 50; no. 1; p. 49
Main Authors Danilova, I G, Shevelev, I V, Isaev-Ivanov, V V, Noskin, L A
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.01.2005
Subjects
Animals
Bacteriophage M13 - genetics
Cattle
Deoxyribonuclease I - metabolism
DNA, Single-Stranded - metabolism
DNA, Viral - metabolism
Lasers
Light
Pancreas - enzymology
Scattering, Radiation
Spectrometry, Fluorescence - methods
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Summary:The effect of catalytic bivalent and inhibitory monovalent cations on the interactions of bovine pancreatic deoxyribonuclease I with the circle single-stranded DNA of M13 phase was studied. It was found that monovalent cations affect the site of binding to DNA and the active center of the enzyme; in their presence, a break of the formation of the enzyme-substrate complex occurs. The results provide evidence that conformational changes of the protein molecule in all cases are more substantial that it could be expected from X-ray data published earlier.
ISSN:0006-3029