Free radical induced damages to rat liver subcellular organelles: inhibition by Andrographis paniculata extract

Aqueous extract of Andrographis paniculata was examined for antioxidant activity using rat liver subcellular organelles as model systems. The study deals with two important biological oxidative agents, ascorbate-Fe(+2) and AAPH generating hydroxyl and peroxyl radical, respectively. Oxidative damage...

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Bibliographic Details
Published inIndian journal of experimental biology Vol. 45; no. 11; p. 959
Main Authors Tripathi, Rakshamani, Kamat, J P
Format Journal Article
LanguageEnglish
Published India 01.11.2007
Subjects
Amidines - pharmacology
Andrographis - chemistry
Animals
Ascorbic Acid - pharmacology
DNA Damage
Female
Free Radical Scavengers - pharmacology
Free Radicals - metabolism
In Vitro Techniques
Liver - cytology
Liver - drug effects
Liver - metabolism
Mice
Microsomes, Liver - drug effects
Microsomes, Liver - metabolism
Oxidants - pharmacology
Oxidative Stress - drug effects
Plant Extracts - pharmacology
Rats
Rats, Wistar
Subcellular Fractions - drug effects
Subcellular Fractions - metabolism
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Summary:Aqueous extract of Andrographis paniculata was examined for antioxidant activity using rat liver subcellular organelles as model systems. The study deals with two important biological oxidative agents, ascorbate-Fe(+2) and AAPH generating hydroxyl and peroxyl radical, respectively. Oxidative damage was examined against the inhibition of membrane peroxidation, protein oxidation and restoration in decreased SOD and catalase activity. The antimutagenic activity of Ap was examined following inhibition in AAPH induced strand breaks in plasmid pBR322 DNA. Extract was a potent scavenger of DPPH, ABTS radicals, exemplified by ESR signals, O2-*, *OH and H2O2, displayed excellent reducing power, FRAP potentials to reduce Fe (III) --> Fe (II) and had considerable amount of phenolics/ flavonoids contents, an effective antioxidant index. The observed antioxidant effect might be primarily due to its high scavenging ability for ROS. Effect was confirmed ex vivo following inhibition in peroxidation, restoration in SOD enzyme, SOD band intensity and protein degradation in Ap fed liver homogenate. Based on these results, it was concluded that the aqueous extract of Andrographis paniculata might emerge as a potent antiradical agent against various pathophysiological oxidants.
ISSN:0019-5189