Cryopreservation of transgenic rat lines

The following protocol is for freezing rat blastocysts by direct plunging into liquid nitrogen. Although this protocol is designed for freezing the embryos in polypropylene cryotubes, it could readily be adapted to 0.5-mL straws. For a general discussion of cryopreservation, the reader is referred t...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 18; p. 269
Main Author Pomeroy, K O
Format Journal Article
LanguageEnglish
Published United States 1993
Subjects
Animals
Blastocyst - cytology
Cryopreservation - methods
Rats
Rats, Transgenic
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Summary:The following protocol is for freezing rat blastocysts by direct plunging into liquid nitrogen. Although this protocol is designed for freezing the embryos in polypropylene cryotubes, it could readily be adapted to 0.5-mL straws. For a general discussion of cryopreservation, the reader is referred to Chapter 25.
ISSN:1940-6029
DOI:10.1385/0-89603-245-0:269