Heat-induced relocalization of protein kinase CK2. Implication of CK2 in the context of cellular stress

Among various other roles described so far, protein kinase CK2 has been involved in cell cycle, proliferation, and development. Here, we show that in response to specific stresses (heat shock or UV irradiation), a pool of the cellular CK2 content relocalizes in a particular nuclear fraction, increas...

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Published inThe Journal of biological chemistry Vol. 275; no. 31; pp. 23919 - 23926
Main Authors Gerber, D A, Souquere-Besse, S, Puvion, F, Dubois, M F, Bensaude, O, Cochet, C
Format Journal Article
LanguageEnglish
Published United States 04.08.2000
Subjects
3T3 Cells
Animals
Biological Transport
Casein Kinase II
Cell Compartmentation
Cell Nucleolus - metabolism
HeLa Cells
Homeostasis
Hot Temperature
Humans
Mice
Oxidative Stress
Protein-Serine-Threonine Kinases - metabolism
Subcellular Fractions
Ultraviolet Rays
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Summary:Among various other roles described so far, protein kinase CK2 has been involved in cell cycle, proliferation, and development. Here, we show that in response to specific stresses (heat shock or UV irradiation), a pool of the cellular CK2 content relocalizes in a particular nuclear fraction, increasing the activity of the kinase there. Electron microscopic analysis shows that upon heat shock, CK2alpha and CK2beta subunits are both detected in similar speckle structures occurring in the interchromatin space but are differentially targeted inside the nucleolus. This CK2 relocalization process takes place in a time- and dose-dependent manner and is reversible upon recovery at 37 degrees C. Altogether, this work suggests CK2 be involved in the response to physiological stress in higher eukaryotic cells.
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ISSN:0021-9258
DOI:10.1074/jbc.M002697200