Multicolor FISH probe sets and their applications

Multicolor fluorescence in situ hybridization (FISH) assays are nowadays indispensable for a precise description of complex chromosomal rearrangements. Routine application of such techniques on human chromosomes started in 1996 with the simultaneous use of all 24 human whole chromosome painting prob...

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Bibliographic Details
Published inHistology and histopathology Vol. 19; no. 1; p. 229
Main Authors Liehr, T, Starke, H, Weise, A, Lehrer, H, Claussen, U
Format Journal Article
LanguageEnglish
Published Spain 01.01.2004
Subjects
Animals
Centromere
Chromosome Banding
Chromosome Painting
Color
Cytogenetics - methods
DNA Probes
Female
Fluorescent Dyes
Genetic Markers
Humans
In Situ Hybridization, Fluorescence - methods
Karyotyping
Ligands
Male
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Summary:Multicolor fluorescence in situ hybridization (FISH) assays are nowadays indispensable for a precise description of complex chromosomal rearrangements. Routine application of such techniques on human chromosomes started in 1996 with the simultaneous use of all 24 human whole chromosome painting probes in multiplex-FISH (M-FISH) and spectral karyotyping (SKY). Since then different approaches for chromosomal differentiation based on multicolor-FISH (mFISH) assays have been described. Predominantly, they have been established to characterize marker chromosomes identified in conventional banding analysis. Their characterization is of high clinical impact and is the requisite condition for further molecular investigations aimed at the identification of disease-related genes. Here we present a review on the available mFISH methods including their advantages, limitations and possible applications.
ISSN:0213-3911
DOI:10.14670/HH-19.229