The influence of omega-3 polyunsaturated fatty acids (omega-3 pufa) on lactobacilli adhesion to the intestinal mucosa and on immunity in gnotobiotic piglets

The effect of application of omega-3 polyunsaturated fatty acids (omega-3 PUFA) on intestinal colonization by Lactobacillus paracasei and on cellular immunity has been investigated in gnotobiotic pigs. The administration of polyunsaturated fatty acids positively affected the adhesion of Lactobacillu...

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Published inBerliner und Münchener tierärztliche Wochenschrift Vol. 116; no. 7-8; p. 312
Main Authors Bomba, Alojz, Nemcová, Radomíra, Gancarcíková, Sona, Herich, Robert, Pistl, Juraj, Révajová, Viera, Jonecová, Zuzana, Bugarský, Andrej, Levkut, Mikulás, Kasteĺ, Rudolf, Baran, Miroslav, Lazar, Gabriel, Hluchý, Marián, Marsálková, Slávka, Posivák, Ján
Format Journal Article
LanguageEnglish
Published Germany 01.07.2003
Subjects
Animals
Bacterial Adhesion - drug effects
Colony Count, Microbial
Fatty Acids, Omega-3 - pharmacology
Female
Germ-Free Life
Intestinal Mucosa - microbiology
Lactobacillus - drug effects
Lactobacillus - growth & development
Lactobacillus - physiology
Probiotics
Random Allocation
Swine - immunology
Swine - microbiology
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Summary:The effect of application of omega-3 polyunsaturated fatty acids (omega-3 PUFA) on intestinal colonization by Lactobacillus paracasei and on cellular immunity has been investigated in gnotobiotic pigs. The administration of polyunsaturated fatty acids positively affected the adhesion of Lactobacillus paracasei to the jejunal mucosa of gnotobiotic piglets. When compared to the control group, the number of Lactobacillus paracasei adhering to the jejunal mucosa was by 12% higher in piglets of the experimental group (5.10 log 10/cm2 vs. 4.55 log 10/cm2). The respective counts of Lactobacillus paracasei adhering to the ileal and colonic mucosa of 28 day old gnotobiotic piglets reached 4.45 and 5.05 log 10/cm2 in group C and 4.44 and 4.95 log 10/cm2 in group E. Omega-3 PUFA supplementation increased the phagocytic activity of neutrophils by almost 100% on day 28 of life as well as the subpopulations of lymphocytes (CD8) in the peripheral blood of germ-free piglets on day 21 of life. Our results indicate that the action of probiotics in the gut may be modulated by dietary PUFA. The stimulatory effect of PUFA upon adhesion of lactobacilli could be used for enhancing the effectiveness of probiotics in inhibiting digestive tract pathogens.
ISSN:0005-9366