New tetrazolium method for the histochemical localization of dipeptidyl peptidase IV

New tetrazolium method for the histochemical localization of dipeptidyl peptidase IV (DPP IV), based on a newly synthesized substrate Gly-L-Pro-1-hydroxy-4-naphthylamide is proposed. Upon the enzyme hydrolysis of the substrate a strong reducing agent, i.e. 4-amino-1-naphthol is released, which reduc...

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Published inCellular and molecular biology (Noisy-le-Grand, France) Vol. 45; no. 2; p. 225
Main Authors Dikov, A, Dimitrova, M, Stoineva, I, Halbhuber, K J
Format Journal Article
LanguageEnglish
Published France 01.03.1999
Subjects
Animals
Dipeptidyl Peptidase 4 - analysis
Female
Indicators and Reagents
Male
Rats
Rats, Wistar
Tetrazolium Salts
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Summary:New tetrazolium method for the histochemical localization of dipeptidyl peptidase IV (DPP IV), based on a newly synthesized substrate Gly-L-Pro-1-hydroxy-4-naphthylamide is proposed. Upon the enzyme hydrolysis of the substrate a strong reducing agent, i.e. 4-amino-1-naphthol is released, which reduces tetrazolium salts to water-insoluble, deeply colored formazans, that precipitate on the sites of enzyme activity, marking them accurately. No auxiliary electron acceptor is needed for the redox reaction. The incubation is performed at the optimal pH of the enzyme. Precise enzyme localization is achieved in all organs studied. Thus, the new method avoids most of the disadvantages of the methods in use and might open new possibilities in peptidases histochemistry.
ISSN:0145-5680