Negative correlation between cyclophilin mRNA level in leukocytes of renal allograft recipients and ciclosporin concentration in whole blood

To study mRNA level of cyclophilin in the white blood cells (WBC) of the renal allograft recipients (RAR) and its correlation with ciclosporin concentration in whole blood. The cyclophilin mRNA levels and beta-actin as controls in the WBC of 47 RAR were measured by quantitative reverse transcription...

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Published inZhōngguó yàolĭ xuébào Vol. 19; no. 3; pp. 276 - 278
Main Authors Zhao, Q, Li, F Q, Wu, J G, Chu, X M, Zhou, Y, Yao, X D
Format Journal Article
LanguageEnglish
Published China 01.05.1998
Subjects
Adolescent
Adult
Cyclosporine - blood
Female
Humans
Immunosuppressive Agents - blood
Kidney Transplantation
Leukocytes - metabolism
Male
Middle Aged
Peptidylprolyl Isomerase - biosynthesis
Peptidylprolyl Isomerase - genetics
RNA, Messenger - genetics
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Summary:To study mRNA level of cyclophilin in the white blood cells (WBC) of the renal allograft recipients (RAR) and its correlation with ciclosporin concentration in whole blood. The cyclophilin mRNA levels and beta-actin as controls in the WBC of 47 RAR were measured by quantitative reverse transcription polymerase chain reaction. The blood ciclosporin assay utilized monoclonal antibody fluorescence polarization immunoassay. With the increase of ciclosporin concentration in whole blood (from 62 micrograms.L-1 to 678 micrograms.L-1), relative cyclophilin mRNA level in the WBC of RAR decreased nonlinearly (from 1.1 to 0.03, r = 0.8195). There was a negative correlation between the mRNA level of cyclophilin in the WBC of RAR and the ciclosporin concentration in whole blood.
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ISSN:0253-9756