Reactivity of alveolar macrophages in lung cancer patients and healthy subjects: surface ICAM-1 after INF-gamma stimulation in vitro

The linearity of ICAM- I expression on alveolar macrophages (AM) before and after INF-gamma stimulation in healthy and lung cancer subjects were compared. AM were collected by bronchoalveolar lavage and incubated with/without INF-gamma according to standard procedures. The harvested cells were analy...

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Published inFolia histochemica et cytobiologica Vol. 40; no. 2; p. 103
Main Authors Hoser, G, Grubek-Jaworska, H, Droszcz, P, Domagała-Kulawik, J, Dabrowska, M, Chazan, R
Format Journal Article
LanguageEnglish
Published Poland 2002
Subjects
Antineoplastic Agents - pharmacology
Bronchoalveolar Lavage Fluid - cytology
Carcinoma, Non-Small-Cell Lung - pathology
Humans
In Vitro Techniques
Intercellular Adhesion Molecule-1 - metabolism
Interferon-gamma - pharmacology
Lung Neoplasms - pathology
Macrophages, Alveolar - drug effects
Macrophages, Alveolar - pathology
Receptors, Cell Surface - drug effects
Stimulation, Chemical
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Summary:The linearity of ICAM- I expression on alveolar macrophages (AM) before and after INF-gamma stimulation in healthy and lung cancer subjects were compared. AM were collected by bronchoalveolar lavage and incubated with/without INF-gamma according to standard procedures. The harvested cells were analyzed by flow cytometry using monoclonal antibodies against leucocytes and macrophages. Only viable cells were analyzed. Stimulation with INF-gamma revealed two AM subpopulations of similar size differentiated in the intensity of ICAM-1 expression. They were not distinctly marked in every studied case. Our preliminary results did not confirm the previously reported decreasing reactivity of AMs after INF-gamma stimulation in lung cancer patients.
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ISSN:0239-8508