Effects of resveratrol and ethanol on production of pro-inflammatory factors from endotoxin activated murine macrophages

• Published in: Acta pharmacologica Sinica Vol. 23; no. 11; p. 1002
• Main Authors: Feng, Yong-Hong, Zou, Jian-Ping, Li, Xiao-Yu
• Format: Journal Article
• Language: English
• Published: United States 01.11.2002
• Subjects: Animals; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Drug Synergism; Endotoxins - pharmacology; Ethanol - pharmacology; Interleukin-1 - biosynthesis; Interleukin-6 - biosynthesis; Macrophage Activation - drug effects; Macrophages, Peritoneal - drug effects; Macrophages, Peritoneal - metabolism; Male; Mice; Mice, Inbred BALB C; Nitric Oxide - biosynthesis; Stilbenes - pharmacology; Tumor Necrosis Factor-alpha - biosynthesis
• ISSN: 1671-4083; 1745-7254

To study the interaction of resveratrol and ethanol on the production of pro-inflammatory factors from activated murine peritoneal macrophages (MPM). NO production was measured with Griess assay; IL-1 production was measured through thymocyte co-stimulating assay; IL-6 and TNF-alpha were detected by ELISA method. Resveratrol (6.25, 12.5, 25 micromol/L) and ethanol (0.2 %, 0.8 %) synergistically inhibited the 24 h production of NO from lipopolysaccharide (LPS, 1 mg/L) and IFN-gamma (5 kU/L) stimulated MPM; resveratrol at higher dose (25 micromol/L) also inhibited IL-6 production. Ethanol additively strengthened this effect. Ethanol had no significant influence on 24 h MPM IL-1 production, but it promoted the ability of resveratrol on enhancing the IL-1 release from activated MPM. Low doses of ethanol inhibited 24 h production of TNF-alpha, however, both dose of ethanol enhanced the promoting effect of resveratrol on TNF-alpha production. Resveratrol and ethanol can interact to influence the production of macrophage function molecules, which is noteworthy in evaluating the health-care effect of wine consumption.