Effect of Misgurnus anguillicaudatus polysaccharide on immune responses of splenocytes in mice

• Published in: Acta pharmacologica Sinica Vol. 23; no. 6; p. 534
• Main Authors: Qin, Chuan-Guang, Huang, Kai-Xun, Xu, Hui-Bi
• Format: Journal Article
• Language: English
• Published: United States 01.06.2002
• Subjects: Adjuvants, Immunologic - pharmacology; Animals; Cell Division - drug effects; Cyclophosphamide; Cypriniformes; Immunocompromised Host; Killer Cells, Natural - drug effects; Killer Cells, Natural - immunology; Mice; Polysaccharides - pharmacology; Spleen - cytology; Spleen - drug effects; Spleen - immunology; T-Lymphocytes - cytology; T-Lymphocytes - drug effects; T-Lymphocytes - immunology; T-Lymphocytes, Cytotoxic - drug effects; T-Lymphocytes, Cytotoxic - immunology
• ISSN: 1671-4083

To investigate the effect of Misgurnus anguillicaudatus polysaccharides (MAP) on immune responses of splenocytes in mice. T lymphocyte proliferation (TLP) was measured by [3H]thymidine incorporation assay. Cytotoxic T lymphocyte (CTL) cytotoxicity and natural killer (NK) activity were determined by release of radioactive chromium [51Cr] from pre-labeled target cells. MAP 5 and 10 mg . kg-1 . d-1, ip for 7 d, could increase TLP, enhance the cytotoxicity of CTL and NK cells, and antagonize the effect of concanavalin A (ConA) on TLP suppressed by cyclophosphamide (CP). Inhibitory rates of CTL cytotoxicity were decreased from 51.4 % in CP control mice to 18.2 % and 35.1 % in MAP-treated CP mice, respectively. Furthermore, administration of MAP 10 and 20 mg . kg-1 . d-1, ip for 7 d, restored the reduced NK cell cytotoxicity caused by CP administration in mice. MAP has protective effect on augmenting T-cell-mediated immunity and NK activity in normal and CP-treated mice.