Effect of cholesterol on the bilayer thickness in unilamellar extruded DLPC and DOPC liposomes: SANS contrast variation study

• Published in: General physiology and biophysics Vol. 23; no. 1; pp. 113 - 128
• Main Authors: Gallová, J, Uhríková, D, Islamov, A, Kuklin, A, Balgavý, P
• Format: Journal Article
• Language: English
• Published: Slovakia 01.03.2004
• Subjects: Cholesterol - analysis; Cholesterol - chemistry; Lipid Bilayers - analysis; Lipid Bilayers - chemistry; Liposomes - analysis; Liposomes - chemistry; Macromolecular Substances - analysis; Macromolecular Substances - chemistry; Membrane Fluidity; Molecular Conformation; Neutron Diffraction - methods; Phase Transition; Phosphatidylcholines - analysis; Phosphatidylcholines - chemistry
• ISSN: 0231-5882

Small-angle neutron scattering on extruded unilamellar vesicles in water was used to study bilayer thickness when cholesterol (CHOL) was added to dilauroylphosphatidylcholine (DLPC) and dioleoylphosphatidylcholine (DOPC) bilayers in molar fraction 0.44. Using the H2O/2H2O contrast variation and the small-angle form of Kratky-Porod approximation, the bilayer gyration radius at infinite contrast R(g,infinity) and the bilayer thickness parameter d(g,infinity) = 12(0.5)R(g,infinity) were obtained at 25 degrees C. Addition of CHOL to DLPC increased the d(g,infinity) from 4.058 +/- 0.028 nm to 4.62 +/- 0.114 nm, while in case of DOPC the d(g,infinity) values were the same in the absence (4.618 +/- 0.148 nm) and in the presence (4.577 +/- 0.144 nm) of CHOL within experimental errors. The role of CHOL-induced changes of bilayer thickness in the protein insertion, orientation and function in membranes is discussed.