The activity of antioxidant enzymes in Arabidopsis thaliana exposed to colchicine and H2O2

Studies on the possible interference of colchicine and H2O2 with the activity of some antioxidant enzymes were carried out on Arabidopsis thaliana v. Columbia grown in Murashige and Skooge nutrient medium. Measurements of superoxide dismutase (SOD), guaiacol peroxidase (POX), ascorbate peroxidase (A...

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Published inCellular & molecular biology letters Vol. 8; no. 3; p. 777
Main Authors Drazkiewicz, Maria, Skózyńska-Polit, Ewa, Wanke, Małgorzata, Swiezewska, Ewa
Format Journal Article
LanguageEnglish
Published England 2003
Subjects
Antioxidants - metabolism
Arabidopsis - cytology
Arabidopsis - enzymology
Ascorbate Peroxidases
Catalase - analysis
Cells, Cultured
Colchicine - pharmacology
Hydrogen Peroxide - pharmacology
Oxidants - pharmacology
Peroxidase - analysis
Peroxidases - analysis
Superoxide Dismutase - analysis
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Summary:Studies on the possible interference of colchicine and H2O2 with the activity of some antioxidant enzymes were carried out on Arabidopsis thaliana v. Columbia grown in Murashige and Skooge nutrient medium. Measurements of superoxide dismutase (SOD), guaiacol peroxidase (POX), ascorbate peroxidase (APX) and catalase (CAT) activities were conducted spectrophotometrically. In the presence of colchicine, SOD activity increased, while CAT, APX and POX activities decreased. Inhibitory H2O2 effects on the activity of the enzymes were found. Colchicine pre-treatment resulted in an increase in CAT activity and a further increase in SOD activity in plants treated with H2O2.
ISSN:1425-8153