Immunohistochemical detection of a very high density lipoprotein (VHDL) in ovarian follicles of Triatoma infestans

The ability of Triatoma infestans ovarian follicles to synthesize a very high-density lipoprotein (VHDL) has been examined by immunohistochemical methods. This kind of lipoprotein can be envisaged as a storage hexameric protein present in the hemolymph of some insect species. VHDL immunoreactivity i...

Full description

Saved in:
Bibliographic Details
Published inBiocell Vol. 25; no. 1; pp. 23 - 27
Main Authors González, M S, Ronderos, J R, Rimoldi, O J, Brenner, R R
Format Journal Article
LanguageEnglish
Published Argentina 01.04.2001
Subjects
Animals
Female
Hemolymph - chemistry
Immunohistochemistry
Insect Proteins - analysis
Lipoproteins, HDL - analysis
Lipoproteins, HDL - isolation & purification
Oocytes - chemistry
Oocytes - cytology
Ovarian Follicle - chemistry
Ovarian Follicle - cytology
Triatoma - chemistry
Online AccessGet full text

Cover

More Information
Summary:The ability of Triatoma infestans ovarian follicles to synthesize a very high-density lipoprotein (VHDL) has been examined by immunohistochemical methods. This kind of lipoprotein can be envisaged as a storage hexameric protein present in the hemolymph of some insect species. VHDL immunoreactivity is observed in oocytes at different stages of maturation. The antigen is present in the oocyte cytoplasm as well as in the follicular epithelial cells. The immunopositive reaction in the apical surface of follicle cells suggests both a VHDL synthesis and a secretion process. Furthermore, VHDL seems to be stored into oocyte in yolk granules. On the contrary, no immunopositive reaction is observed in the intracellular spaces between follicle cells, suggesting that VHDL is not incorporated from hemolymph into the oocyte.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0327-9545