Does the measurement of xanthine oxidase (XOD)/xanthine dehydrogenase (XDH) ratio reflect the actual tissue content of these enzymes?

The purine oxidation via XOD formed from XDH in ischemia in an oxidative or proteolytic way is regarded as a source of reactive O2- species. Usually the XOD/XDH ratio is measured to determine the capacity of tissues to form these important inducers of cell damage. But we found that it is difficult t...

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Bibliographic Details
Published inBiomedica biochimica acta Vol. 48; no. 2-3; p. S114
Main Authors Haberland, A, Schimke, I, Papies, B
Format Journal Article
LanguageEnglish
Published Germany 1989
Subjects
Animals
Biomarkers - analysis
Copper - pharmacology
Dithiothreitol - pharmacology
Edetic Acid - pharmacology
Ketone Oxidoreductases - metabolism
Kinetics
Male
Myocardium - enzymology
Myocardium - pathology
Rats
Rats, Inbred Strains
Xanthine Dehydrogenase - metabolism
Xanthine Oxidase - metabolism
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Summary:The purine oxidation via XOD formed from XDH in ischemia in an oxidative or proteolytic way is regarded as a source of reactive O2- species. Usually the XOD/XDH ratio is measured to determine the capacity of tissues to form these important inducers of cell damage. But we found that it is difficult to fix the in vivo XOD/XDH ratio, because preparation and measurement time and conditions (absence or presence of SH-protecting agent, of cations) influenced this ratio. A supposed additional proteolytic increase of XOD during the killing procedure could not confirmed.
ISSN:0232-766X