Cell surface enzymes of brain cortex cells or lymphocytes during early allogeneic reaction

• Published in: Physiological research Vol. 46; no. 2; pp. 137 - 144
• Main Authors: Kovárů, H, Kovárů, F, Hasková, V
• Format: Journal Article
• Language: English
• Published: Czech Republic 1997
• Subjects: 4-Nitrophenylphosphatase - immunology; 4-Nitrophenylphosphatase - metabolism; Animals; Antibodies, Monoclonal - immunology; Antigen-Presenting Cells - immunology; Cerebral Cortex - enzymology; Cerebral Cortex - immunology; Female; H-2 Antigens - immunology; Lymphocytes - enzymology; Lymphocytes - immunology; Male; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; Mice, Inbred CBA; Rats; Sodium-Potassium-Exchanging ATPase - immunology; Sodium-Potassium-Exchanging ATPase - metabolism; Surface Properties; Tumor Cells, Cultured
• ISSN: 0862-8408

The aim was to study the role of major histocompatibility complex (MHC), in mice named H-2, during early allogeneic reactions (AR) of brain cortex cells or lymphocytes. We used neuronal and glial enriched perikarya, spleen and thymus lymphocytes or their subpopulations. Rat AR was also assayed between C-6 astrocytoma cells and spleen lymphocytes. We demonstrated that: 1) H-2 dependent stimulation of Na+,K+-ATPase and ouabain-sensitive K+-dependent p-nitrophenylphosphatase (K+-pNPPase) activities represented specific response in both AR of unseparated brain cells or lymphocytes. On the other hand, non-specific AR-induced stimulation of Ca2+-ATPase activity was observed. 2) Allogeneic enriched glial fractions reacted similarly by the same enzyme activation in contrast to no change in AR between enriched neuronal fractions. Allorecognition ability of glial cells was confirmed by AR between C-6 astrocytoma cells and lymphocytes. 3) Mature thymus lymphocytes exerted alloreactivity by specific activation of Na+,K+-ATPase or K+-pNPPase, in contrast to no change in AR between immature lymphocyte subpopulations. 4) MHC Class II monoclonal antibody inhibited Na+,K+-ATPase and K+-pNPPase activities in brain cells as well as in thymus and spleen lymphocytes in a dose-dependent manner. Results support former studies about alloantigen-induced uncoupling in brain oxidative cortex metabolism (Kovárů Med. Biol. 58: 273, 1980) via Na+,K+-ATPase and K+-pNPPase inhibition by mechanism which can mimic MHC restriction.