Oxygen free radical scavenging abilities of vitamins C and E, and a grape seed proanthocyanidin extract in vitro

Proanthocyanidins, a group of polyphenolic bioflavonoids, have been reported to exhibit a wide range of biological, pharmacological and chemoprotective properties against oxygen free radicals. We have assessed the concentration-dependent oxygen free radical scavenging abilities of a grape seed proan...

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Bibliographic Details
Published inResearch communications in molecular pathology and pharmacology Vol. 95; no. 2; p. 179
Main Authors Bagchi, D, Garg, A, Krohn, R L, Bagchi, M, Tran, M X, Stohs, S J
Format Journal Article
LanguageEnglish
Published United States 01.02.1997
Subjects
Anthocyanins - pharmacology
Antioxidants - pharmacology
Ascorbic Acid - pharmacology
Catalase - pharmacology
Cytochrome c Group - metabolism
Free Radical Scavengers - pharmacology
Fruit
Hydroxyl Radical - metabolism
In Vitro Techniques
Luminescent Measurements
Mannitol - pharmacology
Plant Extracts - pharmacology
Proanthocyanidins
Seeds - metabolism
Superoxide Dismutase - pharmacology
Superoxides - metabolism
Tocopherols
Vitamin E - analogs & derivatives
Vitamin E - pharmacology
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Summary:Proanthocyanidins, a group of polyphenolic bioflavonoids, have been reported to exhibit a wide range of biological, pharmacological and chemoprotective properties against oxygen free radicals. We have assessed the concentration-dependent oxygen free radical scavenging abilities of a grape seed proanthocyanidin extract (GSPE), vitamin C and vitamin E succinate (VES) as well as superoxide dismutase, catalase and mannitol against biochemically generated superoxide anion and hydroxyl radical using a chemiluminescence assay and cytochrome c reduction. A concentration-dependent inhibition was demonstrated by GSPE. At a 100 mg/l concentration, GSPE exhibited 78-81% inhibition of superoxide anion and hydroxyl radical. Under similar conditions, vitamin C inhibited these two oxygen free radicals by approximately 12-19%, while VES inhibited the two radicals by 36-44%. The combination of superoxide dismutase and catalase inhibited superoxide anion by approximately 83%, while mannitol resulted in an 87% inhibition of hydroxyl radical. The results demonstrate that GSPE is a more potent scavenger of oxygen free radicals as compared to vitamin C and VES.
ISSN:1078-0297