Effect of adenosine on synthesis of pyrimidine nucleotides and RNA in normal lymphocytes and under conditions of chronic lymphoid leukosis

Effect of exogenous adenosine on synthesis of pyrimidine nucleotides and RNA was studied in lymphocytes of the patients with leukemic form of chronic lymphoid leukosis. In presence of adenosine lymphocytes were incubated in mixtures containing the precursors--2,3-2(3)H-aspartic and 2-14C-orotic acid...

Full description

Saved in:
Bibliographic Details
Published inVoprosy meditsinskoi khimii Vol. 27; no. 2; p. 193
Main Authors Filanovskaia, L I, Ushakova, E A, Blinov, M N, Filippova, V N
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.03.1981
Subjects
Adenosine - pharmacology
Aspartic Acid - metabolism
Humans
Leukemia, Lymphoid - metabolism
Leukocytes - metabolism
Lymphocytes - drug effects
Lymphocytes - metabolism
Orotic Acid - metabolism
Pyrimidine Nucleotides - biosynthesis
RNA - biosynthesis
RNA, Neoplasm - biosynthesis
Uridine - metabolism
Online AccessGet more information

Cover

More Information
Summary:Effect of exogenous adenosine on synthesis of pyrimidine nucleotides and RNA was studied in lymphocytes of the patients with leukemic form of chronic lymphoid leukosis. In presence of adenosine lymphocytes were incubated in mixtures containing the precursors--2,3-2(3)H-aspartic and 2-14C-orotic acids as well as 5-3H-uridine. Effect of adenosine on the activity of key enzymes of pyrimidine nucleotide synthesis (orotidine monophosphate pyrophosphorylase and uridine kinase) was studied. The enzymatic activity was estimated using radiochemical procedures in extracts of lymphocytes stored at -20 degrees after acetone drying. Adenosine similarly inhibited synthesis of pyrimidine nucleotides and RNA in normal and leukemic leukocytes. One of the mechanisms, by means of which the inhibitory effect of adenosine was realized, was related to the enzymatic activity of orotidine monophosphate pyrophosphorylase and uridine kinase.
ISSN:0042-8809