Immunocytochemical study of NA+ K(+)-ATPase alpha 1 and beta 1 subunits in human and rat normal hepatocytes using confocal microscopy

Hepatic Na+ K(+)-ATPase was recently shown to be composed of two alpha 1 and beta 1 subunits similar to that of kidney. Its localization on hepatocyte plasma membranes was not clearly established. We have studied the localization of alpha 1 and beta 1 subunits using immunocytochemical method and con...

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Published inCellular and molecular biology (Noisy-le-Grand, France) Vol. 41; no. 4; p. 499
Main Authors Benkoël, L, Benoliel, A M, Brisse, J, Sastre, B, Bongrand, P, Chamlian, A
Format Journal Article
LanguageEnglish
Published France 01.06.1995
Subjects
Animals
Humans
Immunohistochemistry
Liver - enzymology
Male
Microscopy, Confocal
Rats
Rats, Wistar
Sodium-Potassium-Exchanging ATPase - analysis
Sodium-Potassium-Exchanging ATPase - chemistry
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Summary:Hepatic Na+ K(+)-ATPase was recently shown to be composed of two alpha 1 and beta 1 subunits similar to that of kidney. Its localization on hepatocyte plasma membranes was not clearly established. We have studied the localization of alpha 1 and beta 1 subunits using immunocytochemical method and confocal microscopy. In accordance with previous cytochemical findings, the catalytic alpha 1 subunit was distributed in basolateral and bile canalicular membranes of hepatocytes. The beta 1 subunit was not demonstrated, due to its very low amount in the liver. The controversy about the bile canalicular localization was discussed.
ISSN:0145-5680