Fluorescence depolarization studies of melanosomal membranes from different sources

In the present paper we report a comparative study of physical properties and biochemical composition of isolated melanosomal membranes extracted from bovine eyes and from an equine spleen melanoma. Some biophysical characteristics of such membranes were obtained by steady-state and time resolved fl...

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Published inPhysiological chemistry and physics and medical NMR Vol. 17; no. 4; pp. 365 - 370
Main Authors Cavatorta, P, Crippa, P R, Ito, A S, Casali, E, Ferrari, M B, Masotti, L
Format Journal Article
LanguageEnglish
Published United States 1985
Subjects
Animals
Cattle
Cell Membrane - ultrastructure
Eye - ultrastructure
Horse Diseases - pathology
Horses
Melanocytes - ultrastructure
Melanoma - ultrastructure
Melanoma - veterinary
Spectrometry, Fluorescence - methods
Splenic Neoplasms - ultrastructure
Splenic Neoplasms - veterinary
Thermodynamics
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Summary:In the present paper we report a comparative study of physical properties and biochemical composition of isolated melanosomal membranes extracted from bovine eyes and from an equine spleen melanoma. Some biophysical characteristics of such membranes were obtained by steady-state and time resolved fluorescence spectroscopy using DPH as fluorescent probe. By these methods we have measured both static fluorescence polarization and fluorescence lifetimes and from the experimental data we have calculated the rotational correlation times by Perrin's equation. Since dynamic and static parameters, such as fluidity and molecular order, can be determined by these methods, the results are discussed on the basis of the recent theories of the role of the biochemical composition in the molecular structure and properties of membranes.
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ISSN:0748-6642