A dot-immunobinding assay for the serodiagnosis of Pasteurella multocida infection in laboratory rabbits

A dot-immunobinding assay was developed to detect serum IgG specific for lipopolysaccharide of rabbit isolates of P. multocida. The assay detected serum IgG as early as 1 week after experimental subclinical nasal infection, whereas 8 weeks were required to detect antibody by a gel diffusion precipit...

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Bibliographic Details
Published inLaboratory animal science (Chicago) Vol. 37; no. 5; p. 615
Main Authors Manning, P J, Brackee, G, Naasz, M A, DeLong, D, Leary, S L
Format Journal Article
LanguageEnglish
Published United States 01.10.1987
Subjects
Animals
Animals, Laboratory
Cross Reactions
Immunoassay - methods
Immunoglobulin G - analysis
Lipopolysaccharides - immunology
Pasteurella Infections - diagnosis
Pasteurella Infections - immunology
Pasteurella Infections - veterinary
Rabbits
Serologic Tests
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Summary:A dot-immunobinding assay was developed to detect serum IgG specific for lipopolysaccharide of rabbit isolates of P. multocida. The assay detected serum IgG as early as 1 week after experimental subclinical nasal infection, whereas 8 weeks were required to detect antibody by a gel diffusion precipitin test. The assay was more reliable than nasal cultures, in that up to 46% of 16 weekly nasal washings of some infected rabbits failed to yield P. multocida. The bacterial antigen (proteinase k digested cell lysate) used in the assay reacted with IgG that did not cross-react with lipopolysaccharide antigens of B. bronchiseptica, P. pneumotropica or P. hemolytica. The assay is sensitive and specific, easily performed, cost effective, requires no special laboratory instruments and provides a permanent easily stored record.
ISSN:0023-6764