A computer program for modeling the kinetics of gene expression

Enzyme induction may be modeled on the basis of four, quantifiable processes that control the rates at which specific gene products accumulate and decay. These processes include synthesis of functional mRNA, translation and degradation of mRNA, and degradation of the protein product. We present a si...

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Bibliographic Details
Published inBioTechniques Vol. 8; no. 6; p. 654
Main Authors Hargrove, J L, Hulsey, M G, Schmidt, F H, Beale, E G
Format Journal Article
LanguageEnglish
Published England 01.06.1990
Subjects
Animals
Cell Division
Computer Simulation
Enzyme Induction
Gene Expression
Kinetics
Liver - enzymology
Liver - metabolism
Macromolecular Substances
Models, Genetic
Protein Biosynthesis
Proteins - metabolism
Rats
RNA, Messenger - biosynthesis
RNA, Messenger - metabolism
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Summary:Enzyme induction may be modeled on the basis of four, quantifiable processes that control the rates at which specific gene products accumulate and decay. These processes include synthesis of functional mRNA, translation and degradation of mRNA, and degradation of the protein product. We present a simple computer program that permits mathematical simulation of gene expression on the basis of experimentally determined rates of synthesis and degradation. The program was implemented as a spreadsheet using Microsoft Excel for Macintosh and MS-DOS operating systems and also was adapted for HyperCard on the Macintosh. It contains a formula to account for growth of tissue or cell populations. The program predicts amounts of individual mRNAs and proteins (or enzyme activities) in cells as a function of time after a stimulus alters their rates of synthesis or degradation.
ISSN:0736-6205
1940-9818