An improved cytogenetic technique for human solid tumors grown in adhesive-tumor-cell culture system

An improved technique for cytogenetic analysis of primary human solid tumors has been developed using an adhesive-tumor-cell culture system. While the previously described in situ harvesting procedure yielded highly condensed chromosome morphology and inadequate metaphase spread, the newly described...

Full description

Saved in:
Bibliographic Details
Published inAnticancer research Vol. 6; no. 5; p. 973
Main Authors Pathak, S, Hopwood, V, Lee, J S, Tomasovic, B, Spitzer, G
Format Journal Article
LanguageEnglish
Published Greece 01.09.1986
Subjects
Cell Adhesion
Chromosome Banding
Culture Techniques - methods
Humans
Karyotyping
Lung - analysis
Lung Neoplasms - genetics
Metaphase
Neoplasms - genetics
Online AccessGet more information

Cover

More Information
Summary:An improved technique for cytogenetic analysis of primary human solid tumors has been developed using an adhesive-tumor-cell culture system. While the previously described in situ harvesting procedure yielded highly condensed chromosome morphology and inadequate metaphase spread, the newly described harvesting procedures have yielded superior quality metaphase morphology and banding patterns. Cytogenetic analysis was successfully carried out on ten primary lung tumors and eight nonmalignant "normal" lung tissues. This procedure is now routinely used in this laboratory for other solid tumor cytogenetic analysis.
ISSN:0250-7005