The metabolism of arachidonic acid to prostaglandin E2 in plerocercoids of Spirometra erinacei

With a simplified method of extracting and purifying prostaglandins, trace prostaglandins (nanogram order) were detected by gas chromatography-mass spectrometry. Arachidonic acid was metabolized to prostaglandin E2 (PGE2) by plerocercoids of Spirometra erinacei, the PGE2 was detected in the medium a...

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Bibliographic Details
Published inParasitology research (1987) Vol. 79; no. 8; p. 634
Main Authors Fukushima, T, Isobe, A, Hojo, N, Shiwaku, K, Yamane, Y, Torii, M
Format Journal Article
LanguageEnglish
Published Germany 1993
Subjects
Absorption
Albumins - metabolism
Animals
Arachidonic Acid - metabolism
Dinoprostone - biosynthesis
Gas Chromatography-Mass Spectrometry
Snakes - parasitology
Sparganum - immunology
Sparganum - metabolism
Spirometra - immunology
Spirometra - metabolism
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Summary:With a simplified method of extracting and purifying prostaglandins, trace prostaglandins (nanogram order) were detected by gas chromatography-mass spectrometry. Arachidonic acid was metabolized to prostaglandin E2 (PGE2) by plerocercoids of Spirometra erinacei, the PGE2 was detected in the medium after incubation with arachidonic acid, and the role of albumin in the absorption of free arachidonic acid by plerocercoids and in the release of its metabolite was investigated. Plerocercoids absorbed arachidonic acid-binding albumin and released PGE2 efficiently. PGE2 is known to suppress the functions of mononuclear cells of the host. The selective release of PGE2 may be related to the escape mechanism of plerocercoids of S. erinacei from the host immune system to become established larva migrans, i.e., sparganosis.
ISSN:0932-0113
DOI:10.1007/BF00932504