Ascorbate effect on cytokine stimulation of HIV production

We have recently shown that ascorbic acid (AA) suppresses the production of HIV in a latently infected T-lymphocytic cell line (ACH-2) following stimulation with the tumor promoter, PMA. To evaluate the effect of ascorbic acid on virus activation following treatment with inflammatory cytokine, we te...

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Bibliographic Details
Published inNutrition (Burbank, Los Angeles County, Calif.) Vol. 11; no. 5 Suppl; p. 684
Main Authors Harakeh, S, Jariwalla, R J
Format Journal Article
LanguageEnglish
Published United States 01.09.1995
Subjects
Ascorbic Acid - pharmacology
Cell Line
HIV - drug effects
HIV - physiology
Humans
RNA-Directed DNA Polymerase - metabolism
T-Lymphocytes - virology
Tetradecanoylphorbol Acetate - pharmacology
Tumor Necrosis Factor-alpha - pharmacology
Zidovudine - pharmacology
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Summary:We have recently shown that ascorbic acid (AA) suppresses the production of HIV in a latently infected T-lymphocytic cell line (ACH-2) following stimulation with the tumor promoter, PMA. To evaluate the effect of ascorbic acid on virus activation following treatment with inflammatory cytokine, we tested tumor necrosis factor alpha (TNF-alpha) whose levels are elevated in patients with HIV/AIDS. ACH-2 cultures, pretreated with various nontoxic concentrations of ascorbate or AZT were stimulated for 2 h with TNF-alpha, and incubated further with fresh supplements of ascorbate or AZT. At 24 to 48 h post-treatment, the RT activity released into culture supernatant was determined. Results showed that TNF-alpha alone caused approximately 13- to 16-fold stimulation in the level of extracellular RT. Pretreatment with ascorbic acid at 200 micrograms/ml caused a little more than about 2- to 4-fold reduction in extracellular RT levels. Most remarkably, exposure to 300 micrograms/ml ascorbate resulted in approximately 5- to 10-fold lowering of the extra-cellular RT titer. In contrast, no significant suppression in extracellular RT levels was seen with concentrations of AZT in the range of 1-5 micrograms/ml.
ISSN:0899-9007