Evaluation of lymphocytes' influence in luminol-dependent chemiluminescence emission of mouse spleen cells

The focus of this study was to elucidate the influence of lymphocytes on phagocytic cell-dependent chemiluminescence (CL). CL was induced by in vitro addition of mitogens, particulate substances and Keyhole Limpet Hemocyanin (KLH)-antigen. CL was measured in spleen cells of immunologically naive mic...

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Published inMethods and findings in experimental and clinical pharmacology Vol. 8; no. 5; p. 253
Main Authors Müller, S, Fromtling, R A, Fromtling, A M, Pawlak, R, Falkenberg, S, Gillert, K E
Format Journal Article
LanguageEnglish
Published Spain 01.05.1986
Subjects
Animals
Cells, Cultured
Concanavalin A - pharmacology
Female
Hemocyanins - pharmacology
Interleukin-2 - physiology
Luminescent Measurements
Luminol
Lymphocytes - physiology
Male
Mice
Mice, Inbred BALB C
Spleen - cytology
Spleen - physiology
T-Lymphocytes, Helper-Inducer - physiology
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Summary:The focus of this study was to elucidate the influence of lymphocytes on phagocytic cell-dependent chemiluminescence (CL). CL was induced by in vitro addition of mitogens, particulate substances and Keyhole Limpet Hemocyanin (KLH)-antigen. CL was measured in spleen cells of immunologically naive mice and in mice immunized with KLH. Mice immunized with KLH and Freund's adjuvant incomplete (FA) showed a lower CL-response than mice injected with FA only. No influence on CL-activity by lymphocytes derived from immunologically naive mice could be found. Adoptive transfer of T cells from KLH-sensitized donor mice did not influence CL-activity elicited with Zymosan in spleen cells derived from KLH-unsensitized recipient mice. Preincubation of spleen cells with supernatants of T cell cultures or with T cell growth factor (TCGF) stimulated with KLH or Con A could not trigger CL-reactivity. Therefore, the lower CL-response of spleen cells derived from KLH-sensitized mice appears to be not a consequence of a suppressive cellular or lymphokine effect, but is due to direct influence of KLH on accessory cells themselves.
ISSN:0379-0355