Comparative effects of platelet-derived growth factor-BB and insulin-like growth factor-I, individually and in combination, on periodontal regeneration in Macaca fascicularis

• Published in: Journal of periodontal research Vol. 31; no. 5; p. 301
• Main Authors: Giannobile, W V, Hernandez, R A, Finkelman, R D, Ryan, S, Kiritsy, C P, D'Andrea, M, Lynch, S E
• Format: Journal Article
• Language: English
• Published: United States 01.07.1996
• Subjects: Alveolar Process - drug effects; Alveolar Process - physiology; Analysis of Variance; Animals; Becaplermin; Bone Regeneration - drug effects; Drug Combinations; Drug Synergism; Insulin-Like Growth Factor I - pharmacology; Macaca fascicularis; Periodontal Diseases - surgery; Periodontal Ligament - drug effects; Periodontal Ligament - physiology; Platelet-Derived Growth Factor - pharmacology; Proto-Oncogene Proteins c-sis; Recombinant Proteins - pharmacology; Regeneration - drug effects; Statistics, Nonparametric; Wound Healing - drug effects
• ISSN: 0022-3484
• DOI: 10.1111/j.1600-0765.1996.tb00497.x

Platelet-derived growth factor (PDGF) and insulin-like growth factor I (IGF-I) in combination have previously been shown to enhance periodontal regeneration. The objective of this study was to further characterize the biological effects of this combination of growth factors in non-human primates and compare the effects to those of each growth factor individually. Ligature-induced periodontitis was initiated in 10 cynomolgus monkeys. After periodontal lesions were established, surgery was performed, and either a methylcellulose gel vehicle or vehicle containing 10 micrograms each of either PDGF-BB, IGF-I or both PDGF-BB and IGF-I was applied to exposed root surfaces. Biopsies were taken 4 and 12 wk after treatment and the extent of periodontal regeneration was assessed by histomorphometry. At both 4 and 12 wk vehicle-treated lesions generally revealed minimal osseous defect fill (ODF) (8.5 +/- 2.1% and 14.5 +/- 5.7%, respectively) and new attachment (NA) (34.1 +/- 5.2% and 26.6 +/- 10.5%, respectively). IGF-I treatment did not significantly alter healing compared to vehicle in any parameter at both 4 and 12 wk. PDGF-BB-treated sites exhibited significant (p < 0.05) regeneration of NA (69.6 + 12.0%) at 12 wk; trends for PDGF-BB treatment effect were also observed in other parameters at 4 and 12 wk, although these increases were not statistically significant. Treatment with PDGF-BB/IGF-I resulted in 21.6 +/- 5.1% and 42.5 +/- 8.3% ODF at 4 and 12 wk, respectively, and 64.1 +/- 7.7% and 74.6 +/- 7.4% NA at 4 and 12 wk, respectively (all significantly greater than vehicle, p < 0.05). The results from this study demonstrated that: 1) IGF-I alone at the dose tested did not significantly alter periodontal wound healing; 2) PDGF-BB alone significantly stimulated NA, with trends of effect on other parameters; and 3) the PDGF-BB/IGF-I combination resulted in significant increases in NA and ODF above vehicle at both 4 and 12 wk.