Very low density lipoprotein and oxidized very low density lipoprotein induce monocyte chemotactic protein 1 in rabbit aortic smooth muscle cells

• Published in: Chinese medical journal Vol. 109; no. 3; p. 206
• Main Authors: Ruan, Q, Deng, Z, Song, J
• Format: Journal Article
• Language: English
• Published: China 01.03.1996
• Subjects: Animals; Aorta - metabolism; Base Sequence; Chemokine CCL2 - biosynthesis; Gene Expression; Lipoproteins, VLDL - metabolism; Molecular Sequence Data; Muscle, Smooth, Vascular - cytology; Muscle, Smooth, Vascular - metabolism; Oxidation-Reduction; Rabbits; RNA, Messenger - biosynthesis
• ISSN: 0366-6999; 2542-5641

To understand whether very low density lipoprotein (VLDL) and oxidatively modified very low density lipoprotein (OX-VLDL) can stimulate vascular smooth muscle cells (SMCs) to express high level of monocyte chemotactic protein (MCP-1) mRNA. LDL and VLDL were isolated from normal blood donors by density gradient ultracentrifugation and oxidatively modified by adding CuCl2 and incubation at 37 degrees C for 24 hours. Rabbit aortic SMCs were cultured by a substrate-attached explant method, and their total RNA was extracted by guanidinium isothiocyanate method. The expression of MCP-1 mRNA in SMCs was examined by dot blotting analysis using a probe of gamma-22P-end-labeled 35-mer oligonucleotide. Dot blotting analysis showed that cultured SMCs can express MCP-1 mRNA and the level of MCP-1 mRNA was increased significantly in SMCs after 24-hour coculture with VLDL (6-fold) and OX-VLDL (20-fold). VLDL and OX-VLDL both can induce MCP-1 mRNA expression in cultured rabbit aortic SMCs and the latter is more effective than the former.