Oxygen consumption during the indole-3-acetic acid oxidation catalyzed by peroxidase. Effect of the enzyme/substrate ratio

Oxygen consumption during the oxidation of indole-3-acetic acid (IAA) by peroxidase has been studied, in conditions of no limitation by oxygen. For different enzyme/substrate ratios the quotient O2 consumed/IAA oxidized shows rather small differences and it is always less than unity. During the reac...

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Bibliographic Details
Published inRevista espanola de fisiologia Vol. 41; no. 4; p. 401
Main Authors Sabater, F, Acosta, M, del Río, J A, Sánchez-Bravo, J
Format Journal Article
LanguageEnglish
Published Spain 01.12.1985
Subjects
Free Radicals
Horseradish Peroxidase - metabolism
Indoleacetic Acids - metabolism
Kinetics
Oxygen - metabolism
Peroxidases - metabolism
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Summary:Oxygen consumption during the oxidation of indole-3-acetic acid (IAA) by peroxidase has been studied, in conditions of no limitation by oxygen. For different enzyme/substrate ratios the quotient O2 consumed/IAA oxidized shows rather small differences and it is always less than unity. During the reaction, however, the quotient undergoes important variations because oxygen uptake ceases long before the decarboxylation of IAA. The results are discussed in the light of previous proposed mechanisms.
ISSN:0034-9402