Immunodetection of HER-2/neu protein in frozen sections evaluated by image analysis: correlation with overall and disease-free survival in breast carcinomas

The immunodetection of HER-2/neu oncogene product was performed in 108 breast carcinomas using immunoperoxidase technique. Monoclonal anti HER-2/neu protein was applied on frozen sections. Immunoprecipitates were evaluated by a computerized system of image analysis (SAMBA). The percentage of the imm...

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Bibliographic Details
Published inAnticancer research Vol. 13; no. 3; p. 603
Main Authors Charpin, C, Bonnier, P, Devictor, B, Andrac, L, Lavaut, M N, Allasia, C, Piana, L
Format Journal Article
LanguageEnglish
Published Greece 01.05.1993
Subjects
Adult
Aged
Biomarkers, Tumor - analysis
Breast Neoplasms - chemistry
Breast Neoplasms - mortality
Breast Neoplasms - pathology
Carcinoma - chemistry
Carcinoma - mortality
Carcinoma - pathology
Female
Frozen Sections
Humans
Image Processing, Computer-Assisted
Lymphatic Metastasis
Middle Aged
Neoplasm Recurrence, Local - chemistry
Neoplasm Recurrence, Local - mortality
Prognosis
Proto-Oncogene Proteins - analysis
Receptor, ErbB-2
Survival Analysis
Survival Rate
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Summary:The immunodetection of HER-2/neu oncogene product was performed in 108 breast carcinomas using immunoperoxidase technique. Monoclonal anti HER-2/neu protein was applied on frozen sections. Immunoprecipitates were evaluated by a computerized system of image analysis (SAMBA). The percentage of the immunostained tissue surfaces and mean optical densities were correlated with the 5 year overall and disease-free survival rates. It was shown that in optimal conditions of antigen preservation and standardized method of immunoprecipitates evaluation, 67% of breast carcinomas were more than 20% pHER-2/neu positive. The pHER-2/neu overexpression was not significantly correlated with the overall 5 year survival. However large positive anti pHER-2/neu surfaces were correlated with higher risk of recurrence (p = 0.0013) and of metastases (p = 0.035).
ISSN:0250-7005