Diagnostic value of intravenously administered johnin purified protein derivative (PPD) in bovine paratuberculosis

The diagnostic value of intravenously administered johnin purified protein derivative (PPD) was studied in 45 cattle of different age, coming from herds infected by, or free from, Mycobacterium paratuberculosis. In addition to observing the clinical symptoms, the animals' sera were assayed for...

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Published inActa veterinaria Hungarica (Budapest. 1983) Vol. 38; no. 1-2; p. 43
Main Authors Körmendy, B, Tuboly, S, Nagy, G, Török, J, Abonyi, T
Format Journal Article
LanguageEnglish
Published Hungary 1990
Subjects
Animals
Bacterial Proteins - administration & dosage
Cattle
Cattle Diseases - diagnosis
Female
Injections, Intravenous - veterinary
Lymphocyte Activation
Mycobacterium - immunology
Paratuberculosis - diagnosis
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Summary:The diagnostic value of intravenously administered johnin purified protein derivative (PPD) was studied in 45 cattle of different age, coming from herds infected by, or free from, Mycobacterium paratuberculosis. In addition to observing the clinical symptoms, the animals' sera were assayed for specific antibodies by the complement fixation (CFT) and immunodiffusion (AGID) tests. The blastogenic transformation of peripheral lymphocytes was determined on the basis of 3HTdR incorporation. Changes in the neutrophilic leucocyte/lymphocyte ratio of the blood were also monitored. Detection of the pathogen in the faeces was attempted by microscopic examination and by culturing. Combined evaluation of responses elicited by intravenously administered johnin PPD can be a valuable aid in recognizing infected animals, particularly those among the heifer progeny of infected cows.
ISSN:0236-6290