The use of filter paper monoclonal antibodies in a Dot-blot test for typing Neisseria meningitidis B

A simple method for the collection, preservation, shipment, and testing of minute amounts of dried monoclonal antibodies for typing Neisseria meningitidis B is described. The monoclonal antibodies collected on filter paper were extracted in PBS and evaluated by Dot-blot employing whole cells of N. m...

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Bibliographic Details
Published inBrazilian journal of medical and biological research Vol. 27; no. 12; pp. 2889 - 2893
Main Authors De-Gaspari, E N, Ribeiro-Filho, A A, Zollinger, W D
Format Journal Article
LanguageEnglish
Published Brazil 01.12.1994
Subjects
Antibodies, Monoclonal
Bacterial Typing Techniques
Immunoblotting - methods
Neisseria meningitidis - classification
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Summary:A simple method for the collection, preservation, shipment, and testing of minute amounts of dried monoclonal antibodies for typing Neisseria meningitidis B is described. The monoclonal antibodies collected on filter paper were extracted in PBS and evaluated by Dot-blot employing whole cells of N. meningitidis B as antigen. The dried filter paper with monoclonal antibodies could be stored at room temperature for as long as 30 days without detectable changes in antibody response when used for typing outer membrane antigens of N. meningitidis B.
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ISSN:0100-879X