Terminal deoxynucleotidyl transferase activity in the regenerating thymus of x-irradiated mice

• Published in: Thymus Vol. 4; no. 1; p. 45
• Main Authors: Daculsi, R, Astier, T, Legrand, E, Duplan, J F
• Format: Journal Article
• Language: English
• Published: Netherlands 01.01.1982
• Subjects: Animals; Antigens, Surface - analysis; Dexamethasone - pharmacology; DNA Nucleotidylexotransferase - metabolism; DNA Nucleotidylexotransferase - radiation effects; DNA Nucleotidyltransferases - radiation effects; Female; Male; Mice; Mice, Inbred C3H; Regeneration - radiation effects; Thymus Gland - enzymology; Thymus Gland - immunology; Thymus Gland - physiology; Thymus Gland - radiation effects
• ISSN: 0165-6090

The distribution of terminal deoxynucleotidyl transferase (TdT) enzyme activity (EU per 10 8 cells) between peaks I and II was followed for a period of 42 days in regenerating thymus of lethally irradiated (9 Gy) C3H mice restored with 10 6 (C3H x AKR) F1 bone marrow cells. The detection of Thy-1.1 and Thy-1.2 surface antigens allowed for the discrimination between host and donor cells, and the main subpopulations of thymic cells were characterized by their sensitivity to H-2 k antiserum and to dexamethazone. Two peaks of TdT activity could be detected on phosphocellulose chromatographic separation. The distribution of TdT activity between these two peaks was followed during the two periods of thymic endo- and exoregeneration. Peak I TdT activity was closely correlated with the variation in the percentage of high H-2 population. Peak II activity was mostly related to low H-2 cells. The per cell content of both peak I and peak II activities exceeded the norm in rapidly expanding populations. Finally between days 10 and 14 the TdT activity of the endoregenerating population was apparently not different from that of the exoregenerating population between days 14 and 22.