Recombination of epithelial root sheath and dental papilla cells in vitro

Hertwig's epithelial root sheath (HERS) cells were isolated and recombined with ectomesenchymal cells in vitro utilizing extracellular matrix components as substrate. After 14 days in culture, HERS cells were differentiated and exhibited a stratified organization. These features resembled those...

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Bibliographic Details
Published inArchives of medical research Vol. 27; no. 4; p. 573
Main Authors Arzate, H, Portilla Robertson, J, Aguilar Mendoza, M E
Format Journal Article
LanguageEnglish
Published United States 1996
Subjects
Animals
Calcium - analysis
Cell Differentiation - physiology
Cells, Cultured
Cementogenesis
Dental Cementum - cytology
Dental Cementum - metabolism
Dental Enamel - cytology
Dental Papilla - chemistry
Dental Papilla - cytology
Dental Papilla - growth & development
Epithelial Cells
Epithelium - chemistry
Epithelium - growth & development
Extracellular Matrix - metabolism
Mice
Mice, Inbred BALB C
Molar - cytology
Molar - growth & development
Osteopontin
Sialoglycoproteins - analysis
Tooth Calcification - physiology
Tooth Root - chemistry
Tooth Root - cytology
Tooth Root - growth & development
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Summary:Hertwig's epithelial root sheath (HERS) cells were isolated and recombined with ectomesenchymal cells in vitro utilizing extracellular matrix components as substrate. After 14 days in culture, HERS cells were differentiated and exhibited a stratified organization. These features resembled those observed in vivo as epithelial rests of Malassez. A mineralization process was also present in HERS cells, in which calcium salts were deposited. This mineralization was correlated with the strong immunoexpression of osteopontin by HERS. The results obtained add support to the possible role of HERS in the secretion of hypocalcified material on the root during early cementogenesis.
ISSN:0188-4409